Insertion sequences and transposons:

• Types of transposable elements
• Insertion sequences (link to IS element database)
• Replicative transposition
• Non-replicative transposition
• Animation of Tn7 transposition (via Nancy Craig's lab)
• Regulation of transposition
• Phage Mu
• Conjugal transposons
• Constins

Transposon derivatives:

• Some useful Tn10 derivatives (Tn10d)
• Some useful Mu derivatives (Mud)

Portable regions of homology:

• Uses of transposons
• Delivery of transposons
  • Transposition via defective phage
  • Transposition via suicide plasmids
  • Transposition via transposase overexpressed from a plasmid
  • Transposition via transitory expression of transposase
  • Transposition in vitro
• Isolation of random pools of transposon insertions
• Polarity of transposon insertions
• Localized mutagenesis
• Chromosome rearrangements
• In vivo cloning

• Some sample problems about transposition.
• Some sample problems about using transposons as portable regions of homology.

Craig, N., R. Craigie, M. Gellert, and A. Lambowitz. 2002. Mobile DNA II. American Society for Microbiology Press, Washington, DC.

Reznikoff, W. 2003. Tn5 as a model for understanding DNA transposition. Mol Microbiol. 47(5): 1199-1206.

Please send comments, suggestions, or questions to smaloy@sciences.sdsu.edu
Last modified June 14, 2004