In vitro Expression Technology


In vitro expression technology (IVET) is an approach to identify gene products that are turned off in one environment (e.g. in laboratory media) but turned on in a different environment (e.g. during infection of a host). The initial version of IVET was based upon continued expression of a reporter gene in a particular host environment.

The disadvantage of the initial IVET system is that it demands that the gene is expressed throughout growth in a particular environment, so this approach would not detect genes that are transiently turned on during adaptation to a new environment. A more recent version, called RIVET, is based upon a reporter "switch" that results in a permanent inversion.

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Last modified July 16, 2002