Mechanisms of gene regulation

• Bacterial gene regulation
  • Transcriptional repression
  • Transcriptional activation
  • Attenuation
  • Antisense control
  • Quorum sensing (via University of Nottingham)
• Eukaryotic gene regulation

• Operon and gene fusions
• IVET and RIVET
• Signature tagged mutagenesis (STM) and GAMBIT
• Protein-protein interactions

DNA-protein interactions

• Challenge Phage
• SELEX
• Gel shift assays (see Carey, J. 1991. Gel retardation. Methods Enzymol. 208: 103-117)
• DNase Protection Footprinting assays
• Surface plasmon resonance (aka Biacore)

• Some sample problems about use of reporter genes.
• Some sample problems on DNA-protein binding.

Please send comments, suggestions, or questions to smaloy@sciences.sdsu.edu
Last modified April 4, 2006