Mutagen Dose


The amount of mutagenesis caused by a mutagen is determined by three factors: (1) the chemical reactivity of the mutagen with DNA, (2) the concentration of the mutagen and the DNA target, and (3) the amount of time the DNA is exposed to the mutagen. It is typically difficult to control the chemical reactivity of the mutagen, so the dose of a mutagen is usually controlled by varying the concentration of the mutagen or the time of exposure. A few practical considerations to think about when mutagenizing cells are listed below.


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Last modified July 15, 2002