Mutants and Mutations

DNA damage and repair:

• Mechanisms of DNA damage
• Mechanisms of DNA repair
  • Repair of DNA modifications
    • Photolyase
    • Ada
  • Repair of DNA synthesis errors
    • Proofreading by DNA polymerase
    • Mismatch repair by mutHSL
  • Repair of replication fork barriers
    • Recombinational repair
      • recA
      • recFOR
      • recBCD
    • Translesion synthesis: umuCD
• Mutator strains

Mutations:

• Types of mutations
• Missense mutants - properties of amino acids
• Identifying missense mutants
• Frame-shift mutations
• Deletion and insertion mutations
• Mutation rates

Mutagens:

• Examples of some useful mutagens
• Mutagen dose
• The zen of mutagenesis

Mutants:

• Genetic nomenclature
• Strategies for finding mutants
  • Selections
    • Antibiotics
  • Screens
  • Enrichments

• Sample problems on mutation frequency
• Sample problems on types of mutations

Please send comments, suggestions, or questions to smaloy@sciences.sdsu.edu
Last modified March 5, 2003