In vitro genetic tricks
- The scientific revolution
- 1978 Nobel Prize in Medicine to Werner Arber, Ham Smith, and Daniel Nathans for discovering restriction endonucleases
- 1980 Nobel Prize in Chemistry to Paul Berg for developing "recombinant DNA" techniques
- 1980 Nobel Prize in Chemistry to Wally Gilbert and Fred Sanger for developing DNA sequencing techniques
- 1993 Nobel Prize in Chemistry to Kary Mullis for developing PCR
- 1993 Nobel Prize in Chemistry to Michael Smith for developing site-directed mutagenesis
- Oligonucleotide synthesis
- DNA sequencing
- Polymerase chain reaction (PCR)
- Site-directed mutagenesis
- Red swaps - targeted linear recombination in bacteria
- DNA Shuffling (aka Sexual PCR) - generation of new variants by reshuffling gene families
- Some sample problems on DNA cloning and sequencing.
- Some sample problems on restriction mapping.
- Some sample problems on PCR and site-directed mutagenesis.
- Court D, Sawitzke J, Thomason L. 2002. Genetic engineering using homologous recombination.
Annu Rev Genet. 36: 361-88.
- Funnell, B., and G. Phillips. 2004. Plasmid Biology. American Society for Microbiology Press, Washinton DC.
- Poteete A. 2001 What makes the bacteriophage lambda Red system useful for genetic engineering: molecular mechanism and biological function. FEMS Microbiol Lett. 201(1): 9-14.
- Stemmer W. 1994. Rapid evolution of a protein in vitro by DNA shuffling. Nature 370: 389-391.
- Wilkins B. 2002. Plasmid promiscuity: meeting the challenge of DNA immigration control.
Environ Microbiol. 4: 495-500.
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Last modified July 5, 2004