Chromosomes, Genes, and Proteins

• Genome size of some prokaryotes

• Chromosome organization

• DNA - RNA - Protein: A brief review of central dogma
  • The Structure of DNA (an annotated version of Watson and Crick's seminal Nature paper)
  • DNA replication
  • DNA polymerases (link to orst.edu)

  • Template strand vs coding strand

  • Supercoiling
    • Supercoiled plasmid DNA
    • A simple demonstration (link to Univ. Queensland)
    • RNA polymerase reading a negatively supercoiled template

  • Transcription and translation (a cartoon overview)

  • Transcription Initiation
    • Database of E. coli promoters
    • Bioinformatics search for promoters (link to Hanah Margalit's website)

  • Transcription termination
    • Rho-independent transcription termination (via Simon Cawley)
    • Rho dependant polarity

  • Protein synthesis (link to indstate.edu)

  • tRNA and anticodon wobble
  • One gene, one enzyme (usually but not always)

• Some sample problems on DNA, RNA, and protein.
• Some sample problems on prokaryotic chromosomes.

Please send comments, suggestions, or questions to smaloy@sciences.sdsu.edu
Last modified March 5, 2003