Studying Alzheimer's Risk Factors Using iPSC's poster
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Student: William Gaor

Level: Undergraduate
Major: Biology
Host Mentor: Hollis Cline, Ph.D.
Host Institution: Neuroscience, Scrips Research Institute
Date: 2021



Project investigated the risk factor of Alzheimer’s disease, studying the mechanism by which the presence of the allele ε4 of the APOE gene affects astrocyte-neuron interactions. He used Lentiviruses to force the expression of select transcription factors (TF) which can drive the differentiation of human induced pluripotent stem cells (hiPSC) into neurons and astrocytes. Several cell lines of Alzheimer’s disease relevant genotypes with lentiviral vectors that carry specific transcription factors and select, by sub-cloning and then genotyping, the ones that have the integrated virus+TF combination. His role in this project was to generate human iPSC stable lines that carry the transcription factors that will drive their differentiation into neurons and astrocytes.

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