Partial Review list for Biology 555
Several copies of the textbook Scanning and Tramsmission Electron Microscopy by Flegler et al are available in the Reserve Room in Love Library
You might find it useful to review the chapter on Enzyme cytochemistry (pp 283-289) and Immunocytochemistry (pp 263-278) in Electron Microscopy by Bozzola and Russel, also in Reserve Room
We discussed a number of pieces of equipment necessary to prepare or
view samples for an electron microscope. Here is a list of most
of the equipment I diagramed and/or presented in lecture. You are
still responsible for any devices discussed in the text, but not
necessarily presented in lecture.
Diagram the parts, and explain the operation of:
Scanning probe microscope (i.e., Atomic force or scannning
tunneling) (general overview of how one works)
TEM (column and imagers)
SEM (column and detectors)
Gun assembly(including anode plate)
Electromagnetic lens and polepiece
Rotary mechanical pump
Oil diffusion pump
Turbomolecular pump
Titanium sublimation pump
Diffusion-pumped vacuum system
Turbo pumped vacuum system
Slam freezer, propane jet freezer, plunge freezer
Vacuum evaporator(shadowing, replica making)
Ultramicrotome
Sputter coater
Critical Point dryer
EDS detector
WDS detector
Photographic paper or film
Laser printer
Digital interfaces for TEM
Discuss the protocol steps for sample preparation (see also
Bozzolo et alon Reserve List)
SEM microanalysis (EDS or WDS)
TEM pre-embedding immunolabelling
TEM post-embedding immunolabelling