Photography
define as the capture of a luminous image with metallic silver
produced through the reduction of silver halide to metallic silver1834 William Talbot coated paper with sodium chloride, then silver nitrate to form silver nitrate==> produced negative images of overlying leaves etc.
1839 Louis Daguerre coated glass with emulsion==> positive images produced
1885 George Eastman emulsion on cellulose nitrate
Any energy source can be used to form a silver-based image
Composition of recording material:light==>photons, usually less than 10 eV, need several to sensitize a silver grain
SEM==> photons from CRT
TEM==> electrons, usually greater than 20 KeV, need only 1 and can sensitize several grains
paper................................................film
------coating to protect surface------emulsion
AgBr grains (30-2500 nm) in gelatin
gelatin maintains dispersed silver
Latent image formation==>paper -----base----------------- plastic
is chemically imprinted, but not visible, image image formed after exposure but before developmentneed about 10 activated(neutralized) silver atoms to see black grain
exposure of film or paper to photons/electrons produces a latent imageDeveloper== an alkaline reducing solution
Stop Bath== acidic bath to stop the development processover time, the activated silver grain will turn black on ints own. Developer increases the speed of this reaction significantlyreaction time varies with
1) the pH=more alkaline, faster development
2) temperature=higher temperature, faster development
Fixer== removes unexposed silver grains
fixes(coagulates) protein/gelatin into placeconvert the unreduced silver to a water soluble form which is then removed. Without fixing, the silver will turn black
Wash== to remove everything else
residual sulfur compound would form silver sulfide in presence of light and humidity==leaves brown silver sulfide and can convert silver to colorless formPolaroid instant film ==this is an all in one process
developer gel is smeared between print and negative in Positive/Negative formatthe negative film has 10x the resolution of the positive
on negative, sensitized silver is reduced to metallic silver; on positive, have inverse reaction take place
Resolution vs. Film Speed
film or paper is covered
with a layer of silver grains with different sizes
resolution ==smallest measurable distance between two distinct dots
speed ==sensitivity
of film/paper to be exposed
for 10 electrons/photons
the larger the grains, fewer total electronsphotons hitting the film or paper are needed to produce a grain
this would make a faster film/paper
smaller grains, more total electrons/photons hitting the paper or film are needed to produce enough silver grains to form an image
larger grains make for
a grainy image, so resolution is reduced
Spectral sensitivity==wavelengths that material is sensitive to
safelights are relative--won't expose the material in a reasonable length of timefog density baseline density of unexposed filmsilver bromide alone is sensitive to blue, violet and UVto increase this range, additional dyes are linked to the silver grains
orthochromatic film== green sensitized==red insensitive, very high contrastpancromatic film== all wavelengths sensitized==numerous shades of graycan produce an image curve to show rates of exposuregraph density vs. log of exposure
A->B underexposedB->C useful region
C->D over exposed
contrast is function of slope
greater the slope, greater the contrast
PHOTOGRAPHIC PAPERS
Fiber vs. Plastic (Resin coated)
Graded vs. Polycontrast
paper finishes: F glossy, M matte
Magnification
Electronic Capture
1. video or digital camera mounted on light microscopeinstead of film, use electronics and computers
2. SEM== use frame grabber to capture image from analog SEMuse frame-grabber to capture a single frame of videouse computer to average several frames to improve signal to noise
noise is the random spots created by the electronics of the system, not the image
use frame grabber to grab higher resolution slow scan2b. SEM--digital microscopes capture digital image directlyvideo is 30x scans per secslow scan has longer dwell time per spot==better resolution
both of these rely on a YAG (Ytrium Aluminum Garnet) scintillator in detector to convert electron signal to light image and then capture that image
3. TEM==use single crystal YAG (Ytrium Aluminum Garnet) to convert electron signal to light image and then capture that image with a high resolution camera
use a CCD charge coupled device==set of polysilicon electrodes in silicon substrate and separated by an oxide insulation layeradvantage:photons from scintillator hit surface of CCD and raise the electrical potential at each pixel==this is counted by computer and used to form an image
1. dynamic range==ratio of the range of values from the lowest to the highestdisadvantages:for film, it is only 200:1, for CCDs it is 100,000:12. linearity==fully linear for CCD, variable for film3. handling==computer stored, no darkroom 'instant image'
resolution==film is about 10,000 pixels by 10,000 whereas CCDs are 4Kx4K (but getting bigger and cheaper)Examples of image printers:cost==for highest resolution, $90,000 for 2K square chip plus computer , storage, etc. Then need some way to get the image printed
Inkjet printer with a cartridge that shots microdots of colored dye to make spots of image
Laser printer: similar to a xerox machine
laser creates line of charged particles on rotating drum. As the drum passes toner, it attracts colored toner particles onto charged drum. This toner is heat transferred and bonded onto paper, creating an image on the paper (which is why paper from printer or xerox machine feels warm)Dye sublimation: uses colored media that is heated and assigned to a dot of pixel. The amount of color from each of 3 sheets (RGB) determines the final color