EXAM I.
DNA Isolation and Purification
Qiaprep manual (pdf)
DNA isolation protocol (phenol extraction) (pdf)
Genomic DNA isolation from fresh/frozen tissue (pdf)
Agarose Gel-Electrophoresis
Molecular weight markers (NEB)
Restriction Enzyme Mapping
Webcutter 2.0 Free, online software for restriction mapping nucleotide sequences-from Yale Univ. http://www.firstmarket.com/cutter/cut2.htmlEXAM II.Genome Analysis of: Construction of Physical Maps for Linear and Circular Chromosomal DNAs, Determination of Copy Number Ratio and Mapping of Chromosomal Virulence Genes
Katsunori SUZUKI, Kumi IWATA, and Kazuo YOSHIDA http://www.dna-res.kazusa.or.jp/8/4/02/HTMLA/Two Dimensional bacterial genome display (PDF) This paper uses restriction fragment analysis to identify genomic differences between species.
Imaging Restrictions Enzymes and DNA Mapping using the Atomic Force Microscope http://homer.ornl.gov/cbps/mappage.htm
Restriction enzyme mapping tutorial http://faculty.plattsburgh.edu/donald.slish/RestMap/RestMapTutorial.html
Also see problems on Dr. Malloy's web site : www.sci.sdsu.edu/.../problems/ in-vitro/RE-mapping/
Broad host plasmid handouts from Mike Cleary (Kelco) pdf files
Study sheet for Exam II & Answer Key for exam II
EXAM III.
Notes for Exam III
April 15- In Vitro Mutagenesis
April 29- Transgenic animals & plants & cloning
Good website links:
http://www.neb.com/neb/tech/tech_resource/restriction/re_frame.html
setting up a restriction digest-http://www.neb.com/neb/tech/tech_resource/restriction/general/reaction.ht ml
Qiagens handbooks online: http://www.qiagen.com/literature/handbooks/default.asp
This web site has a variety of links to help with database, handbook,
methods and technique resources for Molecular & Cellular Biology:
I don't know who Pedro is (except that he is assistant prof. in Lisbon) but he has put together a great site to find web links for useful programs for molecular research: http://www.public.iastate.edu/~pedro/research_tools.html(look under extras for tutorials)
Don't take the following for granted:http://www3.ncbi.nlm.nih.gov/ this site leads you to PubMed-biomedical literature that searches all recent journals and gives access to abstracts and sometimes entire papers! OMIM- Online Mendelian Inheirtance in Man- reviews all the known inherited diseases; Blast- to search sequences & compares to all known published DNA sequences.