Bio. 551 Rec. DNA
Dr. Michelle Mardahl
Exam II. Study Sheet 40 points:
Topics:
In Vitro Mutagenesis- Reverse Genetics- 25 points
Gene Therapy- 5 points
Transgenic Eggs & Animals- 10 points
Be prepared to use the following to design an experiment to mutate a gene in vitro:
Elimination of restriction site
Linker insertion mutagenesis
Nested Deletions
Dut-ung
Dpn I-
Taq DNA ligase
Cassette Mutagenesis
What are the parameters for primer design for in vitro mutagenesis?
How are mutants screened for using PCR, SSCP?
Terminology- Gene Therapy Approaches
Ex vivo
In situ
In vivo
Chimeraplasty -
Compare & Contrast the advantages & disadvantages of different gene therapy vectors including
a. liposomal
b. viral
c. conjugated DNA
d. transpon-mediated
Compare & contrast the different viral vectors currently in use.
What are scientific & ethical concerns about gene therapy?
Diagram how to make and screen for:
a transgenic fly
transgenic mouse
knock-out mouse
conditional knock-out mouse