10 DNA Fingerprinting + 11 Evolution

1.What would happen if you used one restriction enzyme to cut the DNA found at the crime scene and different ones to cut the DNA of the suspects?

2. Assuming you did everthing correctly, which plates should have live E. coli growing on them? Which one(s) should glow in the dark?

3. What exactly did you need to see to determine if a suspect's DNA matched the crime scene?

4. Why do some DNA fragments migrate farther from their starting point than others in the same lane of the gel?

5. What do you think happens if you load too much DNA into some of the gel lanes (how does it affect your results, if at all)?

6.What did you need to be able to see the bands of DNA?

7.Why did you need to use one restriction enzyme instead of the other to do your "cuts" in the paper simulation of genetic engineering?

8. What will you vary in the computer simulation for next week?

9. What types of selection will you simulate this coming week?

10. Which of these will you use for the next lab? Little paper alleles, dice, cups, beans, simulated moths made out of paper.

11. When a suspect's DNA matches that of the crime scene, what does it mean?