9 Protein Synthesis and Genetic Engineering + 10 DNA Fingerprinting

1.What are"sticky ends" and what purpose do they serve?

2. What is the purpose of the ampicillin antibiotic in some of the agar plates?

3. How do you read the genetic code chart in figure 9.2 and what do the letters represent?

4. What must happen in the nucleus before protein synthesis can occur?

5.Where in the cell does the actual synthesis of proteins occur?

6. What is the "central dogma" of biology?

7.Why did you need to use one restriction enzyme instead of the other to do your "cuts" in the paper simulation of genetic engineering?

8. What bad result might you get if you allowed the innoculating loop, or a pipet tip, to touch the counter top and then continued working with your bacteria?

9. Assuming you did everthing correctly, which plates should have live E. coli growing on them? Which one(s) should glow in the dark?

10. Which of these will you use for the next lab? DNA fragments to put on a gel, paper which we'll cut up to simulate DNA restriction enzymes cutting DNA into fragments, DNA puzzles, UV lights to see the DNA, Computer web site, pH paper

11.How will you know if your DNA has migrated far enough?