| BCMB 1
Laboratory Schedule- Sp 2012 |
|
| Wed. PM, Jan. 18- Fri., Jan. 20 Mon., Jan. 23- Wed. AM, Jan. 25 |
Exercise 1, day 1 (Basic
lab skills)
Class intro, pipetting, making solns, sterile technique Exercise 1, day 2 (Basic lab skills) Excel intro., spectrophotometry |
| Wed. PM, Jan. 25- Fri.,
Jan. 27 Mon., Jan. 30- Wed. AM, Feb. 1 |
Exercise 2, day 1 (Enzyme
kinetics) Alkaline Phosphatase assay with and without inhibitor at different substrate concentrations Exercise 2, day 2 (Enzyme kinetics) Quiz on Ex. 1 (Basic lab skills) AP assay at different enzyme concentrations |
| Wed. P.M, Feb. 1- Fri.,
Feb. 3 Mon., Feb. 6-Wed. AM, Feb. 8 Wed. PM, Feb. 8- Fri., Feb. 10 |
Exercise 3, day 1 (Cell
Fractionation) Fractionation by centrifugation, SDH assay Exercise 3, day 2 (Cell Fractionation) Catalase, acid phosphatase, total protein determination Exercise 3, day 3 (Cell Fractionation) Cell fractionation discussion |
| Mon., Feb. 13- Wed. AM,
Feb. 15 Wed. PM, Feb. 15- Fri., Feb. 17 Mon., Feb. 20- Wed. AM, Feb. 22 Wed. PM, Feb. 22- Fri., Feb. 24 Mon., Feb. 27- Wed. AM, Feb. 29 |
Exercise 4, day 1
(Protein purification) discussion + LDH purification- affinity and anion exchange chromatography Exercise 4, day 2 ( Protein purification) LDH purification cont. (gel filtraiton) and LDH fraction analysis Exercise 4, day 3 (Protein purification) Quiz on Exercise 3 (Cell Fractionation) LDH protein assay, LDH diluted into sample buffer Exercise 4, day 4 (Protein purification) Gel electrophoresis, fix, stain, destain Exercise 4, day 5 (Protein purification) Gel photography and analysis |
| Wed. PM, Feb 29- Fri.,
Mar. 2 Mon., Mar. 5- Wed. AM, Mar. 7 |
Exercise 5, day 1
(Bioinformatics) Exercise 5, day 2 (Bioinformatics) 3T3 cell culturiing (Ex. 6) |
| Wed. PM, Mar. 7- Fri.,
Mar. 9 Mon., Mar. 12- Wed. AM, Mar. 14 Wed. PM, Mar. 14- Fri., Mar. 16 |
Exercise 6, day 1
(Microscopy) Fluorescence labeling, negative staining, phase microscopy, cell fixation, SEM prep Exercise 6, day 2 (Microscopy) EM facility rotation- SEM, TEM, fluorescence Exercise 6, day 3 (Microscopy) Analysis of all samples |
| Mon., Mar. 19-– Wed. AM,
Mar. 21 Wed. PM, Mar, 21- Fri., Mar. 23 Mon., Mar. 26- Fri., March 30 Mon., April 2- Wed. AM, April 4 Wed. PM, April 4- Fri., April 6 |
Exercise 7, day 1 (Basic
cloning methods) PCR (A1-A5); Exercise 7- discussion and lecture Exercise 7, day 2 (Basic cloning methods) Prep of vector DNA and PCR purification, PCR product and vector digest Spring Recess (no labs) Exercise 7, day 3 (Basic cloning methods) gel electrophoresis, isolation of DNA fragments, ligation Exercise 7, day 4 (Basic cloning methods); Exercise 9, day 1 (Genetic mapping) Preparation of competent cells, transformation |
| Mon., April 9- Wed., AM,
April 11 Wed. PM, April 11- Fri., April 13 Mon., April 16- Wed. AM, April 18 |
Exercise 8, day 1
(Restriction mapping)& Exercise 9, day 2 (Genetic mapping) Deterimination of transformation success, colony purification (Ex. 8) Exercise 8, day 2 (Restriction mapping) and Exercise 9, day 3 (Genetic mapping) Plasmid DNA mini-prep and restriction enzyme digests (Ex. 8) Exercise 8, day 3 (Restriction mapping) Gel electrophoresis of restriction digests, making molecular standards curve and determination of plasmid map (Ex. 8) |
| Wed. PM, April 18- Fri.,
April 20 |
Student oral presentations |
| Mon., April 23- Wed., AM,
April 25 |
Quiz on Exercise 9,
locker checkout |