Bio 366L Schedule- Sp 2008
Wed., PM, Jan 23- Fri., Jan. 25 Mon., Jan. 28- Wed. AM, Jan. 30	Exercise 1, day 1 (Basic lab skills) Class intro, pipetting, making solns, sterile technique Exercise 1, day 2 (Basic lab skills) Excel intro., spectrophotometry
Wed, PM, Jan. 30- Fri., Feb. 1 Mon., Feb. 4- Wed. AM, Feb. 6	Exercise 2, day 1 (Enzyme kinetics) Alkaline Phosphatase assay with and without inhibitor at different substrate concentrations Exercise 2, day 2 (Enzyme kinetics) Quiz on Ex. 1 (Basic lab skills) AP assay at different enzyme concentrations
Wed. PM. Feb. 6- Fri., Feb. 8 Mon., Feb. 11- Wed. AM, Feb. 13 Wed. PM, Feb. 13- Fri., Feb. 15	Exercise 3, day 1 (Cell Fractionation) Fractionation by centrifugation, SDH assay Exercise 3, day 2 (Cell Fractionation) Catalase, acid phosphatase, total protein determination Exercise 3, day 3 (Cell Fractionation) Cell fractionation discussion
Mon., Feb. 18- Wed. AM, Feb. 20 Wed. PM, Feb. 20- Fri., Feb. 22 Mon., Feb. 25- Wed. AM, Feb. 27 Wed. PM, Feb. 27- Fri., Feb. 29 Mon., March 3- Wed. AM, March 5	Exercise 4, day 1 (Protein purification) discussion + LDH purification- affinity and anion exchange chromatography Exercise 4, day 2 ( Protein purification) LDH purification cont. (gel filtraiton) and LDH fraction analysis Exercise 4, day 3 (Protein purification) Quiz on Exercise 3 (Cell Fractionation) LDH protein assay, LDH diluted into sample buffer Exercise 4, day 4 (Protein purification) Gel electrophoresis, fix, stain, destain Exercise 4, day 5 (Protein purification) Gel photography and analysis
Wed. PM, March 5- Fri., March 7 Mon., March 10- Wed. AM, March 12	Exercise 5, day 1 (Bioinformatics) Exercise 5, day 2 (Bioinformatics) 3T3 cell  culturiing (Ex. 6)
Wed. PM, March 12- Fri., March 14 Mon., March 17- Wed. AM, March 19 Wed. PM, March 19- Fri., March 21	Exercise 6, day 1 (Microscopy) Fluorescence labeling, negative staining, phase microscopy, cell fixation, SEM prep Exercise 6, day 2 (Microscopy) EM facility rotation- SEM, TEM, fluorescence Exercise 6, day 3 (Microscopy) Analysis of all samples
Mon., March 24- Wed. AM, March 26 Wed. PM, March 26- Fri., March 28 Mon., March 31- Fri., April 4 Mon., April 7- Wed. AM, April 9 Wed. PM, April 9- Fri., April 11	Exercise 7, day 1 (Basic cloning methods) PCR (A1-A5); Exercise 7- discussion and lecture Exercise 7, day 2 (Basic cloning methods) Prep of vector DNA and PCR purification, PCR product and vector digest No Labs (Ceasar Chavez holiday and Spring Break) Exercise 7, day 3 (Basic cloning methods) gel electrophoresis, isolation of DNA fragments, ligation Exercise 7, day 4 (Basic cloning methods); Exercise 9, day 1 (Genetic mapping) Preparation of competent cells, transformation, start viral lysate for genetic mapping experiment, lecture on genetic mapping and 3 factor crosses
Mon., April 14- Wed. AM, April 16 Wed., PM- April 16, Fri., April 18 Mon., April 21- Wed AM, April 23	Exercise 8, day 1 (Restriction mapping)& Exercise 9, day 2 (Genetic mapping) Deterimination of transformation success, colony purification (Ex. 8) Titer phage lysate for  mapping (Ex. 9) Exercise 8, day 2 (Restriction mapping) and Exercise 9, day 3 (Genetic mapping) Plasmid DNA mini-prep and restriction enzyme digests (Ex. 8), Transduction (Ex. 9) Exercise 8, day 3 (Restriction mapping) and Exercise 9, day 4 (Genetic mapping) Gel electrophoresis of restriction digests, making molecular standards curve and determination of plasmid map (Ex. 8), Set up to determine genetic linkage (Ex. 9)
Wed. PM, April 23- Fri., April 25	Exercise 9, day 5 Quiz on Exercise 7 and 8 Calculate genetic linkage Discussion of Exercise 9
Mon., April 28- Wed. AM, April 30	Student oral presentations
Wed. PM, April 30- Fri., May 2	Quiz on Exercise 9, locker checkout