| BCMB 1
Laboratory Schedule- Fall 2009 |
|
| Mon, Aug. 31- Wed. AM, Sept. 2 Wed. PM, Sept. 2- Fri., Sept. 4 |
Exercise 1, day 1 (Basic
lab skills)
Class intro, pipetting, making solns, sterile technique Exercise 1, day 2 (Basic lab skills) Excel intro., spectrophotometry |
| Mon., Sept. 7-Wed. A.M.,
Sept. 9 Wed. P.M., Sept. 9-Fri., Sept. 11 Mon., Sept. 14- Wed. AM, Sept. 16 |
No labs ( Labor Day
holiday) Exercise 2, day 1 (Enzyme kinetics) Alkaline Phosphatase assay with and without inhibitor at different substrate concentrations Exercise 2, day 2 (Enzyme kinetics) Quiz on Ex. 1 (Basic lab skills) AP assay at different enzyme concentrations |
| Wed. P.M, Sept. 16- Fri.,
Sept. 18 Mon., Sept. 21-Wed. AM, Sept. 23 Wed. PM, Sept. 23- Fri., Sept. 25 |
Exercise 3, day 1 (Cell
Fractionation) Fractionation by centrifugation, SDH assay Exercise 3, day 2 (Cell Fractionation) Catalase, acid phosphatase, total protein determination Exercise 3, day 3 (Cell Fractionation) Cell fractionation discussion |
| Mon., Sept. 28- Wed. AM,
Sept. 30 Wed. PM, Sept. 30- Fri., Oct. 2 Mon., Oct. 5- Wed. AM, Oct. 7 Wed. PM, Oct. 7- Fri., Oct. 9 Mon., Oct. 12- Wed. AM, Oct. 14 |
Exercise 4, day 1
(Protein purification) discussion + LDH purification- affinity and anion exchange chromatography Exercise 4, day 2 ( Protein purification) LDH purification cont. (gel filtraiton) and LDH fraction analysis Exercise 4, day 3 (Protein purification) Quiz on Exercise 3 (Cell Fractionation) LDH protein assay, LDH diluted into sample buffer Exercise 4, day 4 (Protein purification) Gel electrophoresis, fix, stain, destain Exercise 4, day 5 (Protein purification) Gel photography and analysis |
| Wed. PM, Oct. 14- Fri.,
Oct. 16 Mon., Oct. 19- Wed. AM, Oct. 21 |
Exercise 5, day 1
(Bioinformatics) Exercise 5, day 2 (Bioinformatics) 3T3 cell culturiing (Ex. 6) |
| Wed. PM, Oct. 21- Fri.,
Oct. 23 Mon., Oct. 26- Wed. AM, Oct. 28 Wed. PM, Oct. 28- Fri., Oct. 30 |
Exercise 6, day 1
(Microscopy) Fluorescence labeling, negative staining, phase microscopy, cell fixation, SEM prep Exercise 6, day 2 (Microscopy) EM facility rotation- SEM, TEM, fluorescence Exercise 6, day 3 (Microscopy) Analysis of all samples |
| Mon., Nov. 2– Wed. AM,
Nov., 4 Wed. PM, Nov., 4- Fri., Nov., 6 Mon., Nov 9- Tues, Nov. 10 (Sections 1,2,4,5 only) Section 3 does this lab Fri., Nov. 13 Wed. AM, Nov. 11 - Thurs., Nov. 12 Mon., Nov. 16- Wed., AM, Nov. 18 |
Exercise 7, day 1 (Basic
cloning methods) PCR (A1-A5); Exercise 7- discussion and lecture Exercise 7, day 2 (Basic cloning methods) Prep of vector DNA and PCR purification, PCR product and vector digest Exercise 7, day 3 (Basic cloning methods) gel electrophoresis, isolation of DNA fragments, ligation Veteran's Day Holiday (Nov. 11) Exercise 7, day 4 (Basic cloning methods); Exercise 9, day 1 (Genetic mapping) Preparation of competent cells, transformation, start viral lysate for genetic mapping experiment, lecture on genetic mapping and 3 factor crosses |
| Wed. PM, Nov. 18- Fri.,
Nov. 20 Mon., Nov. 23- Wed., AM, Nov. 25 Wed. PM, Nov. 25- Fri., Nov. 27 Mon., Nov. 30- Wed. AM, Dec. 2 |
Exercise 8 & 9, day 1
(Restriction mapping)& Exercise 9, day 2 (Genetic mapping) Deterimination of transformation success, colony purification (Ex. 8) Titer phage lysate for mapping (Ex. 9) Exercise 8 & 9, day 2 (Restriction mapping) and Exercise 9, day 3 (Genetic mapping) Plasmid DNA mini-prep and restriction enzyme digests (Ex. 8), Transduction (Ex. 9) Thanksgiving recess (no labs) Exercise 8 &9, day 3 (Restriction mapping) and Exercise 9, day 4 (Genetic mapping) Gel electrophoresis of restriction digests, making molecular standards curve and determination of plasmid map (Ex. 8), Set up to determine genetic linkage (Ex. 9) |
| Wed. PM, Dec. 2- Fri.,
Dec. 4 |
Exercise 9 (Genetic
mapping) Quiz on Exercise 7 and 8 Calculate genetic linkage Discussion of Exercise 9 |
| Mon., Dec. 7- Wed. AM, Dec. 9 | Student oral presentations |
| Wed. PM, Dec. 9-
Fri., Dec. 11 |
Quiz on Exercise 9,
locker checkout |