BCMB
1 Information
Note: The first Bio
366L lab
of
the Fall '09 semester will meet Mon., Aug. 31 at 2:00 PM in NLS 415.
Fall
2009
Section 1- MW 2:00 PM - 4:40 PM
Section 2-MW 6:00 PM-8:40
PM
Section 3- WF 9:00 AM-
11:40 AM
Section 4- TTh 8:00 AM-
10:40 AM
Section 5- TTh 2:00 PM-
4:40 PM
No add list
will
be started before the first scheduled lab. Students who wish to
add
a particular
lab section should attend the first meeting of that section and sign
the add list there. Space may be available
in a section even if the computer says the section is full as enrolled
students may drop or not show up.
Fall 2009
INSTRUCTORS' OFFICE HOURS,
PHONE NUMBERS, AND E-MAIL ADDRESSES
Section 1- Wes Williams
e-mail- wesrwilliams@gmail.com
office
phone- TBA
office hours- TBA
Section 2- Martis Cowles
e-mail- martycowles@hotmail.com
office phone- TBA
office hours- TBA
Section 3- Wesley Burford
e-mail- wesburford@gmail.com
office phone- TBA
office hours- TBA
Section 4- Dana Wilner
e-mail- noodle19@gmail.com
office phone- TBA
office hours- TBA
Section 5- Travis Cottage
e-mail- indytcottage@yahoo.com
office phone- TBA
office hours- TBA
floaters
Section 2- Lorenzo Cipani
e-mail-
cipani@rohan.sdsu.edu
office
phone- TBA
office
hours- TBA
Sections 1 and 5- Kirthika
Haridass
e-mail-
office
phone- TBA
office phone- TBA
Section 3 and 4- Yi-An Lai
e-mail-
MHANB20012001@GMAIL.COM
office
phone- TBA
office phone- TBA
Faculty coordinator for the Bio 366L sections is Dr.
Anca Segall. Her office is located in NLS 327.
Any Bio 366L classroom or grading problems that cannot be
resolved by conferring with a student's TA should be directed to
her.
Grades will be based on 3 written
reports,
3 quizzes, a take-home assignment, an oral presentation, and a
subjective
evaluation. The total number of points possible is 190.
Each
written report will be evaluated, will have a numerical grade assigned,
and will be returned to you. The maximum number of points for
each
quiz, each written report, and the oral presentation is shown in the
table
below.
Weighting
Exercise
1
15 points (quiz)
Exercise
2
20 (written report)
Exercises
3
15 (quiz)
Exercise
4
25 (written report)
Exercise
5
10 (take home assignment)
Exercises
6
20 (report)
Exercises 7 & 8 30 (quiz)
Exercise
9
15 (quiz)
Assigned Exercise 20 (oral presentation)
Subjective
20
Additionally, a subjective evaluation of
your lab work will be made and a maximum of 20 points toward your
overall
grade will be based on this evaluation. The subjective grade will be
assigned
by your laboratory instructor and may be based on factors such as
contribution
to group effort, leadership, enthusiasm, precision in executing
exercises,
following lab rules and safety precautions, clean-up, attendance, and
preparation
for each lab session.
Reports will be considered late if they are not turned in by the end
of the class period on the day they are due. Late reports will be
penalized three points for each day (Monday-Friday) they are late up to
a maximum of 15 points. You may turn in one report up to 2 days
late
without penalty.
Completion of the English Placement Test
and Writing Competency Requirements is required.
Completion of Bio 365 lecture is required
The manual is titled "Biology
366L".
The authors are Segall and Sumnicht. The manual can be
purchased
at KB books located on 5187 College Ave. next to Dominoes
Pizza.
It costs about $ 18.00 and it should be available before the first day
of
classes.
Ex.
3 Ex.
4 Ex.
7
Streak plate procedure
Cell fractionation
URL= http://www.sumanasinc.com/webcontent/anisamples/microbiology/cellfractionation.html
Ion-exchange
chromatography
URL= http://www3.interscience.wiley.com:8100/legacy/college/voet/047119350X/animated_figures/html/6-6.html
Gel
filtration chromatography
URL= http://www3.interscience.wiley.com:8100/legacy/college/voet/047119350X/animated_figures/html/6-9.html
Agarose
Gel
electrophoresis of DNA
URL= http://learn.genetics.utah.edu/units/biotech/gel/
URL= http://www.sumanasinc.com/webcontent/anisamples/majorsbiology/gelelectrophoresis.html
Molecular
Biology
Plasmid
cloning
URL= http://www.sumanasinc.com/webcontent/anisamples/molecularbiology/plasmidcloning_fla.html
Polymerase
Chain Reaction
URL= http://www.sumanasinc.com/webcontent/anisamples/molecularbiology/pcr.html
URL= http://www.sumanasinc.com/webcontent/anisamples/microbiology/streakplate.html
Recent
Press releases:
Gene Helps Regate Longevity in Flies,
and
Perhaps Humans
Dopamine and serotonin, two
neurotransmitters in the central nervous
system, are intimately involved in muscle control, memory, sleep, and
emotional
behavior. They are also linked to illnesses such as Parkinson's disease
and mood disorders. Now, regulation of longevity may be added to this
list.
http://www.ncsu.edu/news/press_releases/03_07/198.htm
Scientists Create Smart Mouse --
Addition
Of Single Gene Improves Learning And Memory
PRINCETON, N.J. -- In an achievement that one day
may give scientists the ability to boost human intelligence, Princeton
University researchers
reported that they have genetically modified
mice to have improved learning and memory.
http://www.princeton.edu/pr/news/99/q3/0902-smart.htm
Study reveals family secret of how
viruses
enter cells
WEST LAFAYETTE, Ind. ? Like sleuths on
the
trail of a family of thieves, scientists have caught another viral
intruder
in action, revealing how two related viruses use similar but distinct
strategies
to enter cells.
http://news.uns.purdue.edu/UNS/html4ever/0001.Rossmann.poliovirus.html
DO YOU LIKE ROLLER COASTER RIDES OR
HATE
THEM? YOUR GENES MAY PLAY A ROLE
WASHINGTON - Attitudes are learned, but
new research shows that differences between people in many attitudes
are
also partly attributable to genetic factors. These include attitudes as
diverse as whether one likes roller coaster rides to controversial
social
issues such as attitudes toward abortion and the death penalty for
murder.
The findings appear in this month's American Psychological
Association's
(APA) Journal of Personality and Social Psychology.
http://www.apa.org/releases/attitudes.html
Princeton Scientists Describe
Genetics
Of Blood Stem Cells
Princeton scientists have outlined the
molecular
genetics behind a great mystery of biology: how blood cells replenish
themselves.
The results -a
database of more than 2,000 genes - give biologists their first
comprehensive
picture of the workings of blood stem cells, the master component of
bone
marrow that gives rise to all cellular constituents of blood, from
red and white cells to platelets.
http://www.princeton.edu/pr/news/00/q2/0602-stemcell.htm
"Gatekeeper" Protein is Key to
Cellular
Life; Links Bacteria, Chloroplasts, and Mitochondria
Columbus, Ohio -Researchers here have
determined
that a seemingly ordinary protein called YidC found within the
membranes
of bacteria serves as a gatekeeper of sorts, allowing into the membrane
other proteins essential for the bacteria to live. When YidC isn't
present,
the bacteria die.
http://www.osu.edu/units/research/archive/dalbey.htm
'Noisy' Genes can have Big Impact
Experiments by Howard Hughes Medical
Institute
(HHMI) investigators have revealed how randomness in gene expression
can
have profound biological effects, both good and bad. The research in
yeast
helps explain how it might be possible for randomness in gene
expression
to lead to differences in cells -- or people for that matter -- that
are
genetically identical.
http://www.hhmi.org/news/oshea.html
New Law
For Resolution Allows Unprecedented Sharpness In Fluorescence
Microscopy
(June 3, 2005)— Researchers from the Max Planck Institute for
biophysical Chemistry in Göttingen establish a new law allowing
unlimited
optical resolution in fluorescence microscopy
Study
reveals major genetic differences between blood and tissue cells
Research by a group of Montreal scientists calls into question one
of the most basic assumptions of human genetics: that when it comes to
DNA, every cell in the body is essentially identical to every other
cell.
http://www.mcgill.ca/newsroom/news/item/?item_id=107673
http://www.sci.sdsu.edu/class/#biology
(biology classes on line)
http://www.sdsu.edu/
(sdsu home page)
http://www.sci.sdsu.edu/cos
(sdsu college of sciences home page)
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi
(PubMed-medline queries)
http://rsb.info.nih.gov/nih-image/index.html
(ImageJ index page)
lab
schedule
writing reports- guidelines
Lodish, H., Baltimore, D., Berk, A.,
Zipursky,
S.L., Matudaira, P., Darnell, J., Molecular Cell
Biology,
Scientific AmericanBooks, Inc., New York, 1995.
Rintoul, D., Weltie, R., Storrie, B.,
Lederman,
M., Student Companion for Molecular Cell Biology,
Scientific
American Books, New York, 1995.
Boyer, R. F., Modern Experimental
Biochemistry,
The Benjamin/Cummings Publishing Co., Inc., 1993.
Voet, Voet and Pratt. Fundamentals of
Biochemistry,
Wiley & Sons, Inc., 1998
To introduce students to fundamental
principles
and operations employed by research labs in the fields of biochemistry
and cell and molecular biology.
Any animals used in the Bio 366l class
are
cared for and handled according to NIH guidelines. At the completion of the Bio
366L course students will be able to: 1.demonstrate
a basic ability to utilize the lab equipment introduced in the class
exercises. 2.explain the basic
principles
underlying the function of lab equipment used in the class. 3.write accurate reports
about
experiments using a standard scientific literature format. 4.explain the purpose behind
techniques and assays used in the lab manual. 5.explain
at a molecular level all procedures, assays, and chemical reactions
presented
in the Bio 366L lab exercises. 6.
perform and explain basic techniques introduced in the lab manual that
are commonly employed
in
biochemistry, cell, and molecular biology laboratories.
7.practice
common lab safety procedures and be able to explain why these
procedures
are important. Photo © PhotoDisc, Inc. Autos
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