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Note:  The first Bio 366L lab of the Fall '09 semester will meet Mon., Aug. 31 at 2:00 PM in NLS 415.

LAB TIMES:

    Fall 2009

Section 1- MW 2:00 PM - 4:40 PM
Section 2-MW 6:00 PM-8:40 PM
Section 3- WF 9:00 AM- 11:40 AM
Section 4- TTh 8:00 AM- 10:40 AM
Section 5- TTh 2:00 PM- 4:40 PM

ADD POLICY

     No add list will be started before the first scheduled lab.  Students who wish to add a particular
lab section should attend the first meeting of that section and sign the add list there.  Space may be available
in a section even if the computer says the section is full as enrolled students may drop or not show up.




INSTRUCTORS' OFFICE HOURS, PHONE NUMBERS, AND E-MAIL ADDRESSES

   Fall 2009

Section 1- Wes Williams
    e-mail- wesrwilliams@gmail.com
    office phone- TBA
    office hours-  TBA

Section 2- Martis Cowles
    e-mail- martycowles@hotmail.com
    office phone- TBA
    office hours- TBA

Section 3- Wesley Burford
    e-mail- wesburford@gmail.com
    office phone- TBA
    office hours- TBA

Section 4- Dana Wilner
     e-mail- noodle19@gmail.com
     office phone- TBA
     office hours-  TBA

Section 5- Travis Cottage
     e-mail- indytcottage@yahoo.com
     office phone- TBA
     office hours- TBA

floaters

Section 2- Lorenzo Cipani
    e-mail-  cipani@rohan.sdsu.edu
    office phone- TBA
    office hours-  TBA

Sections 1 and 5- Kirthika Haridass
    e-mail-
    office phone- TBA
    office phone- TBA

Section 3 and 4- Yi-An Lai
    e-mail- MHANB20012001@GMAIL.COM
    office phone- TBA
    office phone- TBA


Faculty coordinator for the  Bio 366L sections  is  Dr. Anca Segall.  Her office is located in  NLS 327.   Any Bio 366L classroom or grading  problems that cannot be resolved by conferring with a student's  TA should be directed to her.

GRADING

Grades will be based on 3 written reports, 3 quizzes, a take-home assignment, an oral presentation, and a subjective evaluation.  The total number of points possible is 190.  Each written report will be evaluated, will have a numerical grade assigned, and will be returned to you.  The maximum number of points for each quiz, each written report, and the oral presentation is shown in the table below. 

      Weighting

  Exercise  1             15 points (quiz)
  Exercise 2              20 (written report)
  Exercises 3             15 (quiz)
  Exercise 4               25 (written report)
  Exercise 5               10 (take home assignment)
  Exercises 6             20 (report)
  Exercises 7 & 8      30 (quiz)
  Exercise 9               15 (quiz)
  Assigned Exercise   20 (oral presentation)
  Subjective                20 

Additionally, a subjective evaluation of your lab work will be made and a maximum of 20 points toward your overall grade will be based on this evaluation. The subjective grade will be assigned by your laboratory instructor and may be based on factors such as contribution to group effort, leadership, enthusiasm, precision in executing exercises, following lab rules and safety precautions, clean-up, attendance, and preparation for each lab session.
Reports will be considered late if they are not turned in by the end of the class period on the day they are due.  Late reports will be penalized three points for each day (Monday-Friday) they are late up to a maximum of 15 points.  You may turn in one report up to 2 days late without penalty.


PREREQUISITES

Completion of the English Placement Test and Writing Competency  Requirements is required. 

Completion of Bio 365 lecture is required


LAB MANUAL PURCHASE

The manual is titled "Biology 366L".  The authors are Segall and Sumnicht.   The manual can be purchased at KB books located on 5187 College Ave. next  to Dominoes Pizza.  It costs about $ 18.00 and it should be available before the first day of classes.


RELEVANT VIDEO CLIPS

Ex. 3
        Cell fractionation
          URL= http://www.sumanasinc.com/webcontent/anisamples/microbiology/cellfractionation.html

Ex. 4
        Ion-exchange chromatography
          URL= http://www3.interscience.wiley.com:8100/legacy/college/voet/047119350X/animated_figures/html/6-6.html
      
        Gel filtration chromatography
          URL= http://www3.interscience.wiley.com:8100/legacy/college/voet/047119350X/animated_figures/html/6-9.html

Ex. 7
      Agarose Gel electrophoresis of DNA
        URL= http://learn.genetics.utah.edu/units/biotech/gel/
 
        URL= http://www.sumanasinc.com/webcontent/anisamples/majorsbiology/gelelectrophoresis.html

      Molecular Biology
       Plasmid cloning
        URL= http://www.sumanasinc.com/webcontent/anisamples/molecularbiology/plasmidcloning_fla.html

       Polymerase Chain Reaction
         URL= http://www.sumanasinc.com/webcontent/anisamples/molecularbiology/pcr.html

       Streak plate procedure
         URL= http://www.sumanasinc.com/webcontent/anisamples/microbiology/streakplate.html



BCMB INTERESTING ARTICLES

Recent Press releases:

Gene Helps Regate Longevity in Flies, and Perhaps Humans

Dopamine and serotonin, two neurotransmitters in the central nervous system, are intimately involved in muscle control, memory, sleep, and emotional behavior. They are also linked to illnesses such as Parkinson's disease and mood disorders. Now, regulation of longevity may be added to this list.

http://www.ncsu.edu/news/press_releases/03_07/198.htm

Scientists Create Smart Mouse -- Addition Of Single Gene Improves Learning And Memory

    PRINCETON, N.J. -- In an achievement that one day may give scientists the ability to boost human intelligence, Princeton University researchers
    reported that they have genetically modified  mice to have improved learning and memory. 

http://www.princeton.edu/pr/news/99/q3/0902-smart.htm

Study reveals family secret of how viruses enter cells

WEST LAFAYETTE, Ind. ? Like sleuths on the trail of a family of thieves, scientists have caught another viral intruder in action, revealing how two related viruses use similar but distinct strategies to enter cells. 

http://news.uns.purdue.edu/UNS/html4ever/0001.Rossmann.poliovirus.html

  

 DO YOU LIKE ROLLER COASTER RIDES OR HATE THEM? YOUR GENES MAY PLAY A ROLE

  Study With Twins Finds Differences In Certain Attitudes Partly Due To Genetic Factors 

WASHINGTON - Attitudes are learned, but new research shows that differences between people in many attitudes are also partly attributable to genetic factors. These include attitudes as diverse as whether one likes roller coaster rides to controversial social issues such as attitudes toward abortion and the death penalty for murder. The findings appear in this month's American Psychological Association's (APA) Journal of Personality and Social Psychology. 

http://www.apa.org/releases/attitudes.html


Princeton Scientists Describe Genetics Of Blood Stem Cells

Princeton scientists have outlined the molecular genetics behind a great mystery of biology: how blood cells replenish themselves. The results -a
database of more than 2,000 genes - give biologists their first comprehensive picture of the workings of blood stem cells, the master component of bone
marrow that gives rise to all cellular constituents of blood, from red and white cells to platelets. 

http://www.princeton.edu/pr/news/00/q2/0602-stemcell.htm


"Gatekeeper" Protein is Key to Cellular Life; Links Bacteria, Chloroplasts, and Mitochondria

Columbus, Ohio -Researchers here have determined that a seemingly ordinary protein called YidC found within the membranes of bacteria serves as a gatekeeper of sorts, allowing into the membrane other proteins essential for the bacteria to live. When YidC isn't present, the bacteria die. 

http://www.osu.edu/units/research/archive/dalbey.htm

'Noisy' Genes can have Big Impact

Experiments by Howard Hughes Medical Institute (HHMI) investigators have revealed how randomness in gene expression can have profound biological effects, both good and bad. The research in yeast helps explain how it might be possible for randomness in gene expression to lead to differences in cells -- or people for that matter -- that are genetically identical.

http://www.hhmi.org/news/oshea.html


New Law For Resolution Allows Unprecedented Sharpness In Fluorescence Microscopy (June 3, 2005)— Researchers from the Max Planck Institute for biophysical Chemistry in Göttingen establish a new law allowing unlimited optical resolution in fluorescence microscopy

http://www.mpg.de/english/illustrationsDocumentation/documentation/pressReleases/2005/pressRelease20050602/


Study reveals major genetic differences between blood and tissue cells

Research by a group of Montreal scientists calls into question one of the most basic assumptions of human genetics: that when it comes to DNA, every cell in the body is essentially identical to every other cell.

http://www.mcgill.ca/newsroom/news/item/?item_id=107673


OTHER RELEVANT LINKS

http://www.sci.sdsu.edu/class/#biology    (biology classes on line) 

http://www.sdsu.edu/       (sdsu home page) 

http://www.sci.sdsu.edu/cos      (sdsu college of  sciences home page) 

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi    (PubMed-medline queries) 

http://rsb.info.nih.gov/nih-image/index.html   (ImageJ index page)
 


HANDOUTS

lab schedule
 writing reports- guidelines

USEFUL REFERENCES

Lodish, H., Baltimore, D., Berk, A., Zipursky, S.L., Matudaira, P., Darnell,    J., Molecular Cell Biology, Scientific AmericanBooks, Inc., New York,   1995. 

Rintoul, D., Weltie, R., Storrie, B., Lederman, M., Student Companion for    Molecular Cell Biology, Scientific American Books,  New York, 1995. 

Boyer, R. F., Modern Experimental Biochemistry, The Benjamin/Cummings Publishing Co., Inc., 1993. 

Voet, Voet and Pratt. Fundamentals of Biochemistry, Wiley & Sons, Inc.,   1998
 

COURSE PURPOSE

To introduce students to fundamental principles and operations employed by research labs in the fields of biochemistry and cell and molecular biology.
 

ANIMAL USAGE

Any animals used in the Bio 366l class are cared for and handled according to NIH guidelines.
 
 

STUDENT LEARNING OUTCOMES

At the completion of the Bio 366L course students will be able to:

1.demonstrate a basic ability to utilize the lab equipment introduced in the class exercises.

2.explain the basic principles underlying the function of lab equipment used in the class.

3.write accurate reports about experiments using a standard scientific literature format.

4.explain the purpose behind techniques and assays used in the lab manual.

5.explain at a molecular level all procedures, assays, and chemical reactions presented in the Bio 366L lab

exercises.

6. perform and explain basic techniques introduced in the lab manual that are commonly employed

in biochemistry, cell, and molecular biology laboratories.

7.practice common lab safety procedures and be able to explain why these procedures are important.


Photo © PhotoDisc, Inc.


 


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