San Diego State University -- Department of Biology

Curriculum Vitae

Date:  September 26, 2003

Terrence G. Frey

HOME ADDRESS          

4362 Argos Drive
San Diego, California  92116


Department of Biology
San Diego State University
San Diego, California  92182-4614               Phone:
  (619) 594-6756


February 1948


Plain City, Ohio, USA


Married:     Maria A. Huisman     (m. September 23, 1972; dec. July 7, 1989)
                     Beverly K. Barrett     
(October 18, 1998)
   James Eric Frey                    (b. December 1975)
                     Jeremy Ryan Frey
                 (b. May 1980)
                     Jonathan Edward Frey
        (b. December 1981)


1966-70                B.Sc. (Chemistry) The Ohio State University 1970-75

1970-75                 Ph.D. (Biochemistry) University of California, Los Angeles


1977-78   Postdoctoral Research Assistant, Dept of Anatomy, Duke University Medical Center

1975-77    Research Assistant, Microbiology Dept., Biocenter, University of Basel, Switzerland


Sept. 1995 - August 2001   Professor & Associate Chair, Department of Biology, San Diego State University

Sept. 1988 - present            Professor with tenure, Department of Biology, San Diego State University

Aug. 1986 - Sept. 1988      Associate Professor, Department of Biology, San Diego State University

July 1984 - July 1986         Research Associate Professor, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine.

July 1978 - June 1884        Research Assistant Professor, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine.


Lecturer and Co-Course Organizer, Biology 366 "Biochemistry, Cell, and Molecular Biology II," San Diego State University, Spring 2001

Lecturer and Course Coordinator, Biology 200A/202, "Principles of Biology", San Diego State University (Fall 1989/90/98/99/00; Spring 2000).

Lecturer and Course Coordinator, Biology 356, "Cell Biology", San Diego State University
(1991 -- 1995)

Organizer and Primary Lecturer, Biology 750, "Molecular Biophysics," San Diego State University
(1987 -- present).

Guest Lectures:  Molecular Biology 610 "Membrane Protein Structure", Chemistry 740&753 "Electron Microscopy"

Co-Instructor and Co-Course Organizer, Biology 402C/302C, "Chemistry of Living Cells," University of Pennsylvania -- Spring/Summer, 1980-85.

Co-Instructor and Co-Course Organizer, Biology 3C,"Introduction to Biology C," University of Pennsylvania -- Fall, 1983.


Associate Chair, Department of Biology, September 1995 -- August 2001.

Director, College of Sciences Electron Microscopy Facility; August 1986 -- present.

University Senate, SDSU, September 1995 -- May 2001

Cell and Molecular Biology Program Area Curriculum Committee, 1998 -- present

College of Sciences SCEC Advisory Committee, 1999 -- present

SDSU Research Council, 1997-2000

SDSU Committee on Committees and Elections, 1998 -- 2000


Principal Investigator:

Grant-in-Aid #0050769Y “Structure and Function of Mitochondria Revealed by Electron Tomography:  Changes Accompanying Ischaemia and Apoptosis” from the Western States Affiliate of the American Heart Association, July 1, 2000 through June 30, 2002; $59,961 awarded for year 1, $59,975 awarded for year 2.


American Society for Biochemistry and Molecular Biology

American Association for the Advancement of Science

Biophysical Society

Microscopy Society of America

Protein Society

Sigma Xi


Visiting Professor, Neurosciences Department, University of California, San Diego, January 1995 -- September 1995

Member, Biophysical Chemistry Study Section (BBCB), National Institutes of Health, July 1, 1988 -- June 30, 1992.  This committee reviews research grants in Molecular Biophysics and Macromolecular Structure; approximately 90 applications three times each year.

Visiting Scientist, Medical Research Council Laboratory of Molecular Biology, Cambridge, England (Host:  Richard Henderson), July 15 - June 1977, August 10, 1987 and January 8-21, 1989.

Organizer:  "Workshop on Image Processing in Electron Microscopy," held March 24-25 at the University of Pennsylvania, Sponsored by The Pennsylvania Muscle Institute.

ad hoc reviewer of research grants for the Biophysics Program at NSF, the Human Frontier Science Program, and of manuscripts submitted to:  Journal of Molecular Biology, Biochemistry, Biophysical Journal, Journal of Structural Biology,  and Archives of Biochemistry and Biophysics.


Refereed Articles:

Frey, T.G., Perkins, G.A., and Ellisman, M.H. (2006) “Electron Tomography of Intracellular Organelles” Ann. Rev. Biophys. Biomolec. Struct. 35, 1999-224.

A. Ponnuswamy, Nulton,J., Mahaffy, J.M., Salamon, P., Frey, T.G., and Baljon, A.R.C. (2005) “Modeling tubular shapes in the inner mitochondrial membrane” Physical Biology 2, 73-79.

Frey, T.G., Renken, C.S., and Perkins, G.A. (2002) "Insight into mitochondrial structure and function from electron tomography." Biochim. Biophys. Acta 1555, 196-203.

Renken, C.W., Siragusa, G., Perkins, G., Washington, L., Nulton, J., Salamon, P., and Frey, T.G. (2002) "A thermodynamic model describing the nature of the crista junction, a structural motif in the mitochondrion." J. Struct. Biol. 138, 137-144.

Perkins, G.A., Renken, C.W., Frey, T.G., and Elisman, M.H.  "Membrane architecture of mitochondria in neurons of the central nervous system." J. Neurosci. Res. 66, 857-865 (2001)

Perkins, G.A., Renken, C.W., van der Kleij, I.J., Ellisman, M.H., Neupert, W., and Frey, T.G.  "Electron tomography of mitochondria after the arrest of protein import aided by TOM19."  Europ. J. Cell Biol. 80, 139-150 (2001).

Frey, T.G. and Mannella, C.A. "The internal structure of mitochondria."  Trends in Biochemical Sciences 25 319-324 (2000).

Perkins, G.A. and Frey, T.G. "Recent structural insight into mitochondria gained by microscopy." Micron 31, 97-111 (2000).

Frey, P.A. and Frey, T.G. Synthesis of undecagold labeling compounds and their applications in electron microscopic analysis of multiprotein complexes. J. Struct. Biol. 127, 94-100 (1999).

Perkins, G. A., Song, J. Y., Tarsa, L., Deerinck, T. J., Ellisman, M. H. and Frey, T. G.  Electron Tomography of Mitochondria from Brown Adipocytes Reveals Crista Junctions. J. Bioenerg. Biomembr. 30, 431-442 (1998).

Perkins, G., Renken, C., Young, S., Lamont, S., Martone, M., Lindsey, S., Frey, T. and Ellisman, M.  Electron tomography of large, multicomponent biological structures. J. Struct. Biol. 120, 219-227 (1997).

Perkins, G., Renken, C., Martone, M.E., Young, S.J.. Ellisman, M., and Frey, T.  "Electron Tomography of Neuronal Mitochondria:Three-dimensional structure and organization of cristae and membrane contacts"  J. Struct. Biol. 119, 260-272 (1997).

Crum, J., Gruys, K.J., and Frey, T.G.  "Electron microscopy of cytochrome c oxidase crystals:  Labeling of subunit III with a monomaleimide undecagold cluster compound," Biochemistry 33, 13719-13726 (1994).

Frey, T.G. and Murray, J.M.  "Electron Microscopy of Cytochrome c Oxidase Crystals:  Monomer-Dimer Relationship and Cytochrome c Binding Site," J. Mol. Biol. 237, 275-297 (1994).

Frey, T.G.  "Cytochrome c Oxidase:  Structural Studies by Electron Microscopy of Two-Dimensional Crystals," J. Elect. Microsc. Tech. 27, 319-332 (1994).

George, G.N., Cramer, S.P., Frey, T.G., and Prince, R.C.  "X-ray absorption spectroscopy of oriented cytochrome oxidase," Biochim. Biophys. Acta 1142, 240-252 (1993).

Valpuesta, J.M., Henderson, R., and Frey, T.G.  "A cryo-electron microscopic analysis of crystalline cytochrome oxidase," J. Mol. Biol. 214, 237-252 (1990).

Jayaraman, U., Chang, T., Frey, T.G. and Blasie, J.K.  "Electron density profile of two-dimensionally crystalline membranous cytochrome c  oxidase at low resolution," Biophys. J.  51, 475-486 (1987).

Li, Y., Naqui, A., Frey, T.G., and Chance, B.  "A new procedure for purification of monodisperse highly active cytochrome c  oxidase from beef heart,"  Biochemical J. 242, 417-423 (1987).

Renshaw, P.F., Owen,C.S., McLaughlin, A.C., Frey, T.G., Leigh,J.S. Jr."Ferromagnetic contrast agents: a new approach," Magnetic Resonance in Medicine  3, 217-25.(1986)

Frey, T.G., Kuhn, L.A., Leigh, J.S., Costello, M.J. and Chan, S.H.P.  "Cytochrome oxidase:  Structural insights from electron microscopy and from secondary structure prediction," J. Inorg. Biochem. 23, 155-162 (1985).

Frey, T.G. and Eisenberg, D.  "Formation and structure of 1-, 3-, and 6+1-stranded helical cables of glutamine synthetase, Int. J. Biol. Macromol. 6, 2-12 (1984).

Ohnishi, T., LoBrutto, R., Salerno, J.C., Bruckner, R.C., and Frey, T.G.  "Spatial relationships between cytochrome a and a3," J. Biol. Chem. 257, 14821-14825 (1982).

Frey, T.G., Costello, M.J. and Chan, S.H.P.  "Selective contrast in electron microscopy of crystalline cytochrome oxidase," Ultramicroscopy  13, 85-92 (1984).

Costello, M.J. and Frey, T.G.  "Membranous cytochrome c oxidase:  A freeze fracture electron microscopic analysis," J. Mol. Biol. 162, 131-156 (1982).

Frey, T.G., Costello, M.J., Karlsson, B., Haselgrove, J.C., and Leigh, J.S.  "The structure of the cytochrome oxidase dimer:  Electron microscopy of two-dimensional crystals," J. Mol. Biol. 162, 113-130 (1982).

Leibo, S.P., Kellenberger, E., Kellenberger-van der Kamp, C., Frey, T.G., and Steinberg, C.M.  "Gene 24-controlled osmotic shock resistance in bacteriophage T4:  Probable multiple gene functions," J. Virology 30, 327-338 (1979).

Heidner, E.G., Frey, T.G., Held, J., Weissman, L.J., Fenna, F., Lei, M., Harel, M., Sweet, R.M., and Eisenberg, D.  "New crystal forms of glutamine synthetase and implications for the molecular structure," J. Mol. Biol. 122, 163-173 (1978).

Frey, T.G., Chan, S.H.P., and Schatz, G.  "Structure and orientation of cytochrome c oxidase in crystalline membranes:  Studies by electron microscopy and by labeling with subunit-specific antibodies," J. Biol. Chem. 253, 4389-4395 (1978).

Frey, T.G., Eisenberg, D., and Eiserling, F.A.  "Glutamine synthetase forms three- and seven-stranded helical cables," Proc. Natl. Acad. Sci. USA 72, 3402-3406 (1975).

Proceedings - Published Papers and Abstracts:

Siragusa, G., Renken, C, Nulton, J., Frey, T., Salamon, P.  "Biophysics of mitochondria membranes"  Biophys. J. 80, 240a (2001).

Renken C.W., Perkins G.A., Ellisman M.H. and Frey T.  "Crista Junctions Reflect the Osmotic Pressure on the Inner Membrane of Mitochondria." Biophys. J. 78, 140A (2000).

Renken, C.W., Perkins, G.A., Kluck, R.M., Newmeyer, D.D., Ellisman, M.H., and Frey, T.G. Investigation by electron tomography of mitochondrial structure during apoptosis. Biophys. J. 76, A270 (1999).

Perkins, G., Renken, C., Martone, M., Edelman, V., Deerinck, T., Young, S., Ellisman, M., and Frey, T. Biophysical Journal 72, A299 (1997).

Perkins, G., Renken, C., Martone, M., Edelman, V., Deerinck, T., Ellisman, M., and Frey, T. "Mitochondrial structure and compartmentation" 1996 Albany Conference on Frontiers of Mitochondrial Research  (1996).

Renken, C., Perkins, G., Martone, M., Edelman, V., Deerinck, T., Ellisman, M. and Frey, T."Three-dimensional reconstruction of mitochondria by electron tomography," Microscopy Research and Technique  (Abstract) (1996).

Crum,J., K.J. Gruys, P.A. Frey and Frey, T.G.  "Undecagold labeling of cytochrome oxidase:  The site of subunit III identified in two-dimensional crystals," Biophys. J. 66, A8 (1994) Platform presentation of abstract at 1994 meeting of Biophysical Society.

Crum, J., Gruys, K. J. and Frey, T. G.  Undecagold labeling of cytochrome c oxidase dimer crystals. Proceedings of the 49th Annual Meeting of the Electron Microscopy Society of America., San Francisco Press, Inc. (1991).

George, G.N., Cramer, S.P., Frey, T.G., and Prince, R.C.  "X-ray absorption studies of oriented cy­tochrome oxidase," in Advances in Membrane Biochemistry and Bioenergetics, (Kim, C.H., Tedeschi, H., Diwan, J.J., and Salerno, J.C., eds.) Plenum Pub. Corp. (1988) pp 429-438.

Frey, T.G. and Chang, T., "The shape of cytochrome c  oxidase and its position in the lipid bilayer."  Ann. N.Y. Acad Sci. 483, 120-130 (1986).

Chang, T. and Frey, T.G., "Sectioning grids of negatively stained cytochrome oxidase crystals," Proc. 45th Ann. EMSA Meeting, Albuquerque, NM 172-173 (1986).

Costello, M.J., Fetter, R.D., and Frey, T.G.  "Complementary replicas of ultra-rapidly frozen specimens," Science of Biological Specimen Preparation, (Muller,M., Becker, R.P., Boyde, A. and Wolosewick, J.J. eds.) SEM Inc., AMF O'Hare, Chicago, pp 95-101 (1986).

Frey, T.G., Costello, M.J., Reardon, J.E. and Frey, P.A.  "Selective contrast in images of crystalline cytochrome oxidase dimers," 41st Ann. EMSA Meeting, Phoenix, Ariz. 442-443 (1983).

Shuman, H., Somlyo, A.V., Safer, D., Frey, T.G., Somlyo, A.P., "Applications of energy filtered imaging in biology," Scanning Electron Microsc. (Pt 2), 737-43 (1983).

Shuman, H., Somlyo, A.V., Somlyo, A.P., Frey, T.G., and Safer, D.  "Energy loss imaging in biology," 40th Ann. EMSA Meeting, Washington, D.C., pp 416-419 (1982).

Frey, T.G., Karlsson, B., Uberbacher, E., Haselgrove, J., and Leigh, J.S.  "The structure of the matrix side of beef heart mitochondrial cytochrome c oxidase," in Symposium on Interaction Between Iron and Proteins in Oxygen and Electron Transport (C. Ho ed.) Elsevier/North Holland, New York, pp 145-150 (1982).

Frey, T.G. and Chan, S.H.P.  "The structure of cytochrome c oxidase:  Immuno-electron microscopy and image analysis of two-dimensional crystals," in Electron Microscopy at Molecular Dimensions.  State of the Art and Strategies for the Future (W. Baumeister and W. Vogell eds.) Springer-Verlag, Heidelberg, pp 81-90 (1980).

Frey, T.G., Chan, S.H.P., and Schatz, G.  "The structure and orientation of cytochrome oxidase in crystalline vesicles," in Frontiers of Biological Energetics:  From Electrons to Tissues Vol. II(P.L. Dutton, J.S. Leigh, and A. Scarpa, eds.) Academic Press, New York, , pp 817-824 (1979).

Frey, T.G., Eisenberg, D., and Smith, P.R.  "The variation of intermolecular contacts in helical aggregates of glutamine synthetase," Proc. 9th International Congress on Electron Microscopy, Vol. II, pp 174-175 (1978)

Frey, T.G.  "The structures of glutamine synthetase and cytochrome c  oxidase--Studies by electron microscopy and image analysis," Proc. 9th International Congress on Electron Microscopy, Vol. III, State of the Art Symposia, pp 107-119 (1978).

Saltzgaber, J., Cabral, R., Birchmeier, W., Kohler, C., Frey, T., and Schatz, G.  "The assembly of mitochondria," in International Cell Biology 1976-1977, (B.R. Brinkley and K. R. Porter, eds.) The Rockefeller University Press, New York, pp 256-263 (1977).

Cabral, R., Birchmeier, W., Deters, D., Frey, T., Kohler, C., and Schatz, G. "Structure and biosynthesis of cytochrome c oxidase," in Genetics and Biogenesis of Chloroplasts and Mitochondria, (Th. Bucher, W. Neupert, W. Sebald, and S. Werner, eds.) Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1976).

Weiss, R., Frey, T.G., Burton, Z., Eiserling, F.A. and Eisenberg, D.  "Models for helical cables of glutamine synthetase and refinement of the models to electron microscopic data by least squares," Proc. 6th European Congress on Electron Microscopy, Vol. I, 20-23 (1976).

Book Chapters and Reviews:

Frey, T.G. "Structural study of heme proteins by electron microscopy of 2-dimensional crystals."  in Analytical Methods for Chlorophyll, Heme and Related Molecules, Witty, M. and Smith, A. eds. (in press) BioTechniques Books, Natick, MA.

Perkins, G. and Frey, T. "Confocal Microscopy" in Encyclopedia of Microbiology, vol. 3, pp 264-275 Lederberg, J. ed. (2000).

Perkins, G. and Frey, T. "Optical Microscopy" in Encyclopedia of Microbiology, vol. 3, Lederberg, J. ed., pp 288-306 (2000).

Perkins, G and Frey, T.  Microscopy in Encyclopedia of Molecular Biology, Tom Creighton, ed., (1999).

Costello, M.J. and Frey, T.G.  "Membrane proteins of the mitochondrion," in Electron Microscopy of Proteins Vol. 6 (J.R. Harris and R.W. Horne eds.) Academic Press Inc., London (1987) pp 377-443.