Mitochondrion & ER Reconstructed by Electron Tomography

Author: T. G. Frey


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Three-dimensional reconstruction of a dendritic mitochondrion from chick cerebellum reconstructed tomographically from a tilt series of a 0.5 micron section. The tomogram was segmented by manually contouring into regions bounded by the outer and inner boundary membranes shown in dark blue and light blue respectively. Four different cristae are displayed in red, yellow, green and grey along with two segments of endoplasmic reticulum in green and light brown. The outer membrane and the inner boundary membrane are rendered translucent so that the cristal membranes can be viewed through it. Note that the inner boundary membrane and the cristal membrane are components of the inner mitochondrial membrane and are one continuous surface joined at 30 nm diameter tubular connections or crista junctions.

Ref: Perkins, G., Renken, C., Martone, M.E., Young, S.J., Ellisman, M., and Frey, T. "Electron Tomography of Neuronal Mitochondria: THree-Dimensional Structure and Organization of Cristae and Membrane Contacts" Journal of Structural Biology 119, 260-272 (1997).