TERM PAPER INSTRUCTIONS

Term papers will be due in my office by 10:00 am May 26, the Tuesday after finals . You should select a technique with some Biophysical relevance, research the topic, and write a 10 - 20 page term paper (double spaced or space and a half). The topic of your paper can (but needn't) be related to one we've covered in class, but if so it should extend beyond what has been presented. You should clear your choice of topic with me before putting too much work into it. The paper can (and probably should) include some figures, and should contain the following elements.

INTRODUCTION:

THEORETICAL BASIS: An explanation of the physical basis of the technique including equations if relevant.

EXPERIMENTAL: What are the experimental requirements? This includes the type and function of each piece of equipment and the requirements of the sample which will be measured etc.

RESULTS: What type of data does one obtain from the experiment, how is it interpreted, and what can it tell you?

DISCUSSION: Whatever you feel is appropriate.

Some possible topics are:

FRAP - fluorescence recovery after photobleaching

Measuring protein secondary structure by spectroscopy (IR, Raman, NMR etc.

Fluorescence Ratio Imaging (to determine e.g. intracellular [Ca++])

Capillary Electrophoresis

Computer Analysis of Two-dimensional Electrophoresis Gels

NMR Imaging

Protein Structure Determination by NMR

Scanning Confocal Light Microscopy

Mass Spectrometry of Proteins

The Three-Dimensional Structure of Biological Macromolecules by Cryo-Electron Microscopy May emphasize: spherical viruses; membrane proteins etc.

Analytical Electron Microscopy

Scanning Probe Microscopy: e.g. scanning tunneling microscopy, atomic force microscopy, etc.

But don't feel that you're limited to these choices.