The Guide to Phage Genomics: Isolation and Purification of your Phage Particles

Guide Home Phage isolation and purification Clone library construction Sequencing Assembly Post-assembly analyses	The Guide to Phage Genomics: Isolation and Purification
	OVERVIEW Isolate and plaque purify phage Grow up to get ~ 10^12 phage and harvest the lysate PEG precipitate to concentrate the phage Isolate the phage particles using CsCl (equilibrium or step gradient) Pull the phage band and dialyze against a buffer Extract the phage DNA
	Forest's Phage Protocols Cesium Chloride Prep of Phage Community DNA SYBR Gold Stain of Phage and Bacteria Tangential Flow Filtration of Phage Concentration Experiments Matt's Cyanophage Protocols Plating, plaque purification, CsCl purification, DNA extraction

Page created: February 20, 2003 Last modified: February 25, 2003 For questions or comments, e-mail Forest Rohwer forest@sunstroke.sdsu.edu or Matt Sullivan mbsulli@mit.edu